Ontogenesis of myosin light chain phosphorylation in guinea pig tracheal smooth muscle
نویسندگان
چکیده
منابع مشابه
Myosin light chain kinase phosphorylation in tracheal smooth muscle.
Purified myosin light chain kinase from smooth muscle is phosphorylated by cyclic AMP-dependent protein kinase, protein kinase C, and the multifunctional calmodulin-dependent protein kinase II. Because phosphorylation in a specific site (site A) by any one of these kinases desensitizes myosin light chain kinase to activation by Ca2+/calmodulin, kinase phosphorylation could play an important rol...
متن کاملThe role of myosin light chain kinase phosphorylation in beta-adrenergic relaxation of tracheal smooth muscle.
Myosin light chain kinase from smooth muscle has been shown to be phosphorylated by cyclic AMP-dependent protein kinase, which leads to a decrease in the affinity of the kinase for Ca2+ . calmodulin and, hence, a decrease in enzymatic activity. This event has been proposed as a mechanism for the relaxation of smooth muscle in response to increased intracellular concentrations of cyclic AMP. The...
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The stimulatory effect of the extract of Portulaca oleracea (P. olerace) on β-adrenoceptor of tracheal smooth muscle was examined. To examine β-adrenoceptor stimulatory effect, concentration response curve to isoprenaline was obtained in pre-contracted tracheal smooth muscle in the presence of three concentrations of aqueous-ethanolic extract, propranolol and saline. Values of EC50 (the effecti...
متن کاملRegulation of myosin light chain and phosphorylase phosphorylation in tracheal smooth muscle.
The activity of phosphorylase kinase and myosin light chain kinase can be regulated by calcium-calmodulin or cyclic AMP. Since phosphorylation of the 20,000dalton phosphorylatable light chain (P-light chain) of myosin may be involved in the regulation of contractile activity in smooth muscle, we examined the relationship between P-light chain phosphorylation, phosphorylase a formation, and isom...
متن کاملQuantitation of myosin light chain phosphorylation in intact smooth muscle.
An improved method for quantitating the extent of myosin light chain (P-LC) phosphorylation in small smooth muscle samples is described. Native myosin was isolated from other cellular proteins in a crude supernatant fraction prepared from a few milligrams of bovine tracheal smooth muscle by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium pyrophosphate (PPi). When potassium i...
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ژورنال
عنوان ژورنال: Pediatric Pulmonology
سال: 2005
ISSN: 8755-6863,1099-0496
DOI: 10.1002/ppul.20150